Wnt Signaling Phospho Antibody Array
Wnt signaling has a direct association with cancer, type II diabetes, and neurodiseases. Signaling of the highly secreted glycolipoproteins in the Wnt family controls embryonic development and adult homeostasis by regulating β-Catenin’s accumulation in the cytoplasm. When Wnt signaling is absent, β-Catenin is targeted for ubiquitination and degraded by the destruction complex formed by Axin, APC, GSK3, PP2P, and CK-1α. As a result, β-Catenin cannot accumulate in the cytoplasm. When Wnt binds to Frizzled receptors and LRP5/6, Wnt signaling is turned on and disrupts the destruction complex’s function. Now β-Catenin accumulates, moves into the nucleus, and binds to TCF/LEF transcriptor factors to activate gene transcription.
Wnt Signaling Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. It is suitable for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine and serine/threonine phosphorylation at specific sites.
Key Features
- Site-specific phosphorylation profiling and screening
- Suitable samples include: cell lysates; frozen or FFPE tissue lysates
- Glass based array with high specificity and low background
- Antibodies covalently attached to 3D polymer coated glass surface
- Sensitive fluorescent detection
User’s Guide
Antibody Array User’s Guide
Product Details
| Number of Antibodies: | 227 site-specific and phospho-specific antibodies; 6 replicates per antibody |
| Targets: | Array Antibody List |
| Reactivity: | Human: 100%; Mouse: 92%; Rat: 80% |
| Internal Controls: | beta-actin; GAPDH; Negative controls |
| Detection Method: | Fluorescence; Compatible Scanners |
| Slide dimensions: | 76 x 25 x 1 mm |
| Product Size: | 2 array slides per package for analyzing two samples (control vs. treated) |
| Storage Condition: | 4°C for 6 months |
Additional Reagent Needed:
Antibody Array Assay Kit
$350.00
